PAGESDS Laemmli Protocol

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所属分类:蛋白质组学

 

Stock Solutions1) 1.5M TrisHCl pH 8.3 + 0.4% SDS (adjust pH before you add the SDS). Keep RT.
2) 30% Acrylamide 0.8% Methylene bis Acrylamide or 40% Acrylamide / Methylene bis Acrylamide (ratio: 37.5:1). Keep 4°C
3) 0.5M TrisHCl pH 6.8+ 0.4% SDS (adjust pH before you add the SDS). Keep RT.
4) 10% Ammonium Persulfate (APS). Keep 4°C less than 1 month.
Running Buffer x5  

Trizma 0.125M 15.1gr Glycine 0.96M  72.0gr  SDS 0.5% 5.0gr H2O up to   1 liter

Keep at RT

Dissolving (Sample) Buffer x5
 

Glycerol 5ml SDS 1gr ß Mercapto Ethanol
(or 0.25M DTT) 2.56ml 0.5M TrisHCl pH 6.8+ 0.4% SDS  2.13ml Bromo Phenol Blue traces

Keep in aliquots of 1ml at -20C

 

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