请教质谱高人啊,这结果怎么分析啊

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所属分类:蛋白质谱技术

我的蛋白样品到上海基康公司去作简单质谱分析,利用的叫maldi-tof技术(激光解吸电离-飞行时间),可以分析样品的肽指纹图谱,他们还通过源后衰变(post souce decay,PSD)来获得一级结构数据。
我得到了结果,但我实在不太懂怎么分析。看了本站的一些讨论,还是有点稀里糊涂。因为我的样品是个未知蛋白,所以我不知道哪个匹配结果可信,可信度也没什么概念。像下面这个,55的得分,27%的覆盖相似性,说明得了多少问题呢?(附件有我的结果,可信件与我联系,3183229@sina.com)
Match to: P12217 Score: 55 Expect: 0.046
(ACCD_MARPO) Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (EC 6.4.1.2) (ACCase beta chain) Acetyl-coenzyme A carboxylase carboxyl transferas

Nominal mass (Mr): 35803; Calculated pI value: 8.77
NCBI BLAST search of P12217 against nr
Unformatted sequence string for pasting into other applications

Taxonomy: Marchantia polymorpha

Variable modifications: Oxidation (M),Pyro-glu (N-term E)
Cleavage by Trypsin: cuts C-term side of KR unless next residue is P
Number of mass values searched: 46
Number of mass values matched: 10
Sequence Coverage: 27%

Matched peptides shown in Bold Red

1 MSLMNWFEDK RRFGGLIGAF IEKATKGYIF SEREKDRYIK IDTTKGLWTR
51 CDNCENMLYV RFLRQNKRIC EECGYHLQMS STERIELLID RGTWYPMDED
101 MTARDVLKFS DEDSYKNRIA FYQKRTGLTD AIQTGIGQLN GIPIALGVMD
151 FQFMGGSMGS VVGEKITRLI EYATRASMPL IIVCSSGGAR MQEGTLSLMQ
201 MAKISSVLQI HQAQKRLLYI AILTYPTTGG VTASFGMLGD IIIAEPKAYI
251 AFAGKRVIEQ TLRQKIPDGF QVAESLFDHG LLDLIVPRNL LKGVLSEIFE
301 LYNAAPCKKF QNSFFK

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