扩增子分析QIIME2-4分析实战Moving Pictures

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所属分类:生物信息学

# 按BodySite分组,统计unweighted_unifrace距离的组间是否有显著差异
qiime diversity beta-group-significance \  --i-distance-matrix core-metrics-results/unweighted_unifrac_distance_matrix.qza \  --m-metadata-file sample-metadata.tsv \  --m-metadata-category BodySite \  --o-visualization core-metrics-results/unweighted-unifrac-body-site-significance.qzv \  --p-pairwise
 
# 按Subject分组,统计unweighted_unifrace距离的组间是否有显著差异
qiime diversity beta-group-significance \  --i-distance-matrix core-metrics-results/unweighted_unifrac_distance_matrix.qza \  --m-metadata-file sample-metadata.tsv \  --m-metadata-category Subject \  --o-visualization core-metrics-results/unweighted-unifrac-subject-group-significance.qzv \  --p-pairwise
 
# 可视化三维展示unweighted-unifrac的主坐标轴分析
qiime emperor plot \  --i-pcoa core-metrics-results/unweighted_unifrac_pcoa_results.qza \  --m-metadata-file sample-metadata.tsv \  --p-custom-axis DaysSinceExperimentStart \  --o-visualization core-metrics-results/unweighted-unifrac-emperor.qzv
 
# 可视化三维展示bray-curtis的主坐标轴分析
qiime emperor plot \  --i-pcoa core-metrics-results/bray_curtis_pcoa_results.qza \  --m-metadata-file sample-metadata.tsv \  --p-custom-axis DaysSinceExperimentStart \  --o-visualization core-metrics-results/bray-curtis-emperor.qzv
 
# 网页展示结果,或下载在线查看
qiime tools view core-metrics-results/bray-curtis-emperor.qzv

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